연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.
1. Experimental Background
Aminopeptidase N (APN) is a Zn2+ dependent exopeptidase found in the plasma membranes, small intestinal and renal membranes. APN preferentially hydrolyzes N-terminal neutral amino acids of proteins and peptides. The structural features of APN include the active site within a large cellular ectodomain, a single transmembrane domain and a short N-terminal cytoplasmic domain.
Based on the observations of APN activity assay, as the concentration of substrate increases the APN activity is greater and hence the fluorescence levels are increased (Fig. 1, 3). This direct correlation pattern must be true because APN is more likely to bind and react in presence of higher substrate concentration leading to greater enzymatic activities.
"Aminopeptidase N (CD13) as a Target for Cancer Chemotherapy." Wickstrom 2011 - Cancer Science - Wiley Online Library. Web. 29 Nov. 2016.
Basic Molecular Biotechnology Laboratory Booklet Department of Biotech. Yonsei University.
"Biology 401: Enzyme Inhibition and Regulation." Biology 401: Enzyme Inhibition and Regulation. Web. 29 Nov. 2016.
"Enzyme Kinetics." Enzyme Kinetics | Chemistry@TutorVista.com. Web. 29 Nov. 2016.
Nelson, David L., Albert L. Lehninger, and Michael M. Cox. Lehninger Principles of Biochemistry. New York: W.H. Freeman, 2008. Print.