연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.
2. Experimental Background
Protein purification is a technique used to isolate a single protein type that is usually extracted from a sample of biological tissue or complex mixture such as a cell lysate. Many times, single specific protein interest is caught in a matrix of other protein or even non-protein parts and hence protein purification must be done to separate the single desired proteins from all other mixtures.
AKTA FPLC purification of His-tagged protein was performed and following curves were attained after applying the samples through immobilized Ni-columns. 10mM binding buffer & 500mM elution buffer (with imidazole) were used.
Basic Molecular Biotechnology Laboratory Booklet – Department of Biotech.Yonsei University.
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"Guidelines for Protein Purification at Low Temperature." GE Healthcare Life Sciences. General Electric Company, Aug. 2015. Web. 20 Nov. 2016. <http://www.gelifesciences.com/>.
"Imidazole." ACS Reagent, ≥99% (titration) | C3H4N2 | Sigma-Aldrich. Web. 22 Nov. 2016. <http://www.sigmaaldrich.com/catalog/product/sial/i2399?lang=ko® ion=KR>.
Man, Protein. "What Are Protease Inhibitors and How Do They Work?" What Are Protease Inhibitors and How Do They Work? Web. 20 Nov. 2016. <http://info.gbiosciences.com/blog/bid/160009/What-are-Protease-Inhibitorsand-How-Do-They-Work>.