검색어 입력폼

에드만실험법

저작시기 2009.10 |등록일 2010.01.21 한글파일한글 (hwp) | 3페이지 | 가격 300원

소개글

에드만분해법에 대해 조사한 자료입니다.

목차

[Mechanism]
[Limitations]

본문내용

[Mechanism]
Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. [1] In this method, the amino-terminal residue is labeled and cleaved from the peptide without disrupting the peptide bonds between other amino acid residues.

Phenylisothiocyanate is reacted with an uncharged terminal amino group, under mildly alkaline conditions, to form a cyclical phenylthiocarbamoyl derivative. Then, under acidic conditions, this derivative of the terminal amino acid is cleaved as a thiazolinone derivative. The thiazolinone amino acid is then selectively extracted into an organic solvent and treated with acid to form the more stable phenylthiohydantoin (PTH)- amino acid derivative that can be identified by using chromatography or electrophoresis. This procedure can then be repeated again to identify the next amino acid. A major drawback to this technique is that the peptides being sequenced in this manner cannot have more than 50 to 60 residues (and in practice, under 30).
다운로드 맨위로