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HPLC 이론 발표 자료

저작시기 2008.07 |등록일 2009.04.13 파일확장자어도비 PDF (pdf) | 20페이지 | 가격 4,000원

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HPLC에 대해서 간략한 이론과 함께 심화 내용까지 일괄적으로

목차

Principle of Chromatographic Separation
Classification
Column Efficiency
Effective peak number216
van DeemterEquation
Eddy, Longitudinal Diffusion
InterphaseMass Transfer, Overall
For HPLC
HPLC
Mobile Phase Injection System
.
.

본문내용

Principle of Chromatographic Separation
◎ Equilibrium of solutes between a stationary phase and mobile phase
◎ solute moving down the column
at a different rate determines their
equil. between the two phase
◎ by separation mechanism
① Adsorption chromatography
② Partition chromatography
③ Ion exchange chromatography
④ Size exclusion chromatography
Classification
① Adsorption
-Stationary phase:
solid
- Sorption &
desorption process
③ Ion exchange
-Stationary phase:
ion exchange resin
- Ion exchange equil.
④ Size exclusion
-Stationary phase:
porous solid
- Also known as GPC
② Partition
- Stationary phase:
liquid on inert solid
- Mobile phase:
liquid (LLC)
gas (GLC)
Column Efficiency
◎ Theoretical plate
- plate height (H) = length of column / # of theoretical plates
- # of theoretical plates (= efficiency)
◎ For high efficiency
① plate height ↓ = # of theoretical plates ↑
② narrower the peak (wb ↓) = # of theoretical plates ↑
③ # of theoretical plates ↑ = greater resolution
Effective peak number
van DeemterEquation
◎ Chromatography efficiency

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