위 자료는 피부조직 수복용 재료로써 콜라겐-히알루론산 다공성 복합재료의 응용가능성을 연구한 자료입니다.
2. Materials and methods
2.1. Fabrication and crosslinking of porous collagen–hyaluronic acid matrix
2.2. Morphological characterization of crosslinked collagen matrices containing various amounts of HA
2.3. HA retention in crosslinked collagen–HA matrices and water uptake ability
2.4. Cell culture
2.5. Cell attachment and proliferation testing
2.6. Cell migration test
2.7. In vivo animal testing
3. Results and discussion
HA content and water uptake after cross-linking
Pore size analysis
Degradation rate of EDC-crosslinked collagen–HA matrices
Cell attachment analysis by MTT assay
Cell attachment analysis by SEM
Cell proliferation profile
Histological observation of cell proliferation
Migration of fetal human dermal fibroblasts from cell populated matrices (CPMs)
Evaluation of wound healing
• Porous collagen–HA matrix containing interconnected pores with mean diameters of 150–250 um was fabricated by freeze-drying and EDC cross-linking.
• In culture conditions, the HA concentration in the matrices decreased slowly after an initial rapid decrease.
• Collagen–HA matrices containing 5.4% or 9.6% HA increased migrating cell numbers significantly.
• In animal testing, however, no significant effect of HA upon wound size reduction was found.
Si-Nae Park, Hye Jung Lee, Kwang Hoon Lee, Hwal Suh, Biological characterization of EDC-crosslinked collagen–hyaluronic acid matrix in dermal tissue restoration, Biomaterials, 24, 1631–1641 (2003)