- Sources and importance of phosphorus
- Existing flow techniques
- Analysis of phosphorus by means of flow techniques
- Case studies
1. Spectrophotometric determination of phosphate and silicate by sequential injection using molybdenum blue chemistry
2. Development of an enzymatic flow-injection chemiluminescence system for determining inorganic pyrophosphate ion
Phosphorus detection methods are classified into optical and electrochemical techniques.
The optical techniques are based on UV-vis spectrophotometry, fluorescence, chemiluminescence, or some other methods. UV-vis spectrophotometric methods show the most wide range of determinable concentration, and chemiluminescence photometric methods have low detection limits and high sensitivity. Among the flow methods using electrochemical detection, the potentiometric and amperometric methods have been the most widely used for phosphorus analysis.
In the large number of publications, FIA and UV–vis spectrosphotometric detection methods have been used to analyze phosphorus in different types of samples. This can be explained in terms of the ease of implementation of FIA. Also, UV–vis spectrophotometric detection is easy to implement and inexpensive.
And the PMB method is the most widely used procedure for the determination of P. The ease of implementation of the on-line digestion, preconcentration, separation systems or enzymatic reactors in FIA systems have allowed the spread of the use of the PMB method to all types of samples. The PMB method is so well established in the field of the analysis of phosphorus that is constitutes the reference method for new ones.