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[FPLC] FPLC

등록일 2004.06.07 파워포인트파일MS 파워포인트 (ppt) | 27페이지 | 가격 2,000원

소개글

powerpoint 슬라이드 문서입니다.
FPLC에대한 발표자료입니다.
영어로 작성되어있습니다.

목차

FPLC-Liquid Chromatography System for Fast Protein Purification

HPLC-High Performance Liquid Chromatography

Base Material for HPLC
Gel Filtration Chromatography
IEC, AC & HIC Chromatography
IEC, AC & HIC Chromatography

Chromatography Methods
Four Major Properties
Four Classic Chromatography

Gel Filtration Chromatography

Ion Exchange Chromatography
Characteristics
IEC media
Functional groups

Hydrophobic Interaction Chromatography
Characteristics
HIC media
Hydrophobic Ligands

Affinity Chromatography
Characteristics
AC media
Coupling to NHS-activated Gel
Polyhistidine tagged fusion protein purification using chelating gel
Human IgG purification using Protein A gel

Purification Strategy

Application

본문내용

Ion Exchange Chromatography
Characteristics
- Change of surface charge with solution pH
NH2 -> NH3+, COOH -> COO-
pI : isoelectric point, net charge is zero.
- Cationic proteins are bound to cation exchange gel.
- Elution : gradient or step methods with high salt buffer

IEC media
- Base material : agarose (Sepharose), cellulose (Sephacell), synthetic polymer
- Manufacturer : Pharmacia, Millipore, Waters, Bio-Rad
- Strong & weak ion exchangers
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