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Selection of medium and Serum
Ham's F12 was designed for Chinese hamster ovary cells
DMEM was developed for mouse fibroblasts for transformation and viru propagation studies. 2X amino acid conc., 4X vitamin conc., 2X HCO3- and CO2 conc. than BME.
αMEM has additional AA, vit, nuclosides, lipoic acid
Ham's F12 with DMEM 50:50v/v be used for many primary culture,
for more fastidious cell lines
RPMI1640 was developed for lymphoid cells and attached cell (weak Ca2+)
Fischer's was developed for L5178Y lymphoma
L15 medium is used as a transport and primary culture (due to absence of HCO3- and CO2)
animal cell culture